By H. Wagner (auth.), Hildebert Wagner, Gudrun Ulrich-Merzenich (eds.)
After the winning advent of acupuncture to the West, fresh advances in analytical tools in chemistry, molecular biology and structures biology – in particular the improvement of the “omic” applied sciences – have back introduced chinese language medications into the point of interest of analysis on conventional chinese language medication (TCM). With greater than a thousand guides at the chemistry, molecular biology and pharmacology of TCM medications in foreign journals over the past 10 years, chinese language medicines are gaining more and more recognition and impression. those information provide nice possibilities for the improvement of latest prescribed drugs for varied medical purposes. foreign scientists have compiled appropriate and development atmosphere study leads to this booklet. subject matters diversity from the newest tools of caliber and defense insurance by means of chemical and genetic fingerprints to the advance of recent prescription drugs for a destiny evidence-based treatment e.g. for melanoma, cardiovascular, inflammatory or infectious ailments in addition to to fresh experimental effects on multitarget and synergy study for the guidance of multi-extract-pharmaceuticals from TCM.
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Otherwise microscopic examination of drugs requires botanical expertise for the unequivocal authentication, as related species often possess similar features. With the improvements in molecular biotechnology and plant genetics in the past decades, genetic tools are considered to provide more reliability for authentication of herbal materials at the DNA level (Kumar et al. 2009). Thus in the meanwhile, various DNA-based molecular marker techniques are applied in many fields and their application is remarkably increasing for species characterization in medicinal plants (Shaw et al.
In general, these primer pairs produce polymorphic PCR fragments from the species of origin and their close relatives, but transportability to more distant taxa is limited. Attempts to design universal primers to amplify chloroplast microsatellites have 2 DNA-Based Authentication of TCM-Plants: Current Progress and Future Perspectives 39 resulted in a set of consensus chloroplast microsatellite primers (ccmp1–ccmp10) that aims at amplifying cpSSR regions in the chloroplast genome of dicotyledonous angiosperms (Weising and Gardner 1999).
It is estimated that 70–80 % of people worldwide rely chiefly on traditional herbal remedies as their primary form of health care (Farnsworth and Soejarto 1991; Pei 2001). Herbal medicine is becoming more main-stream and fashionale in richer countries. Therefore the TCM market has been growing at 10–20 % annually in Europe and North America over recent years (Hamilton 2003; Ten Kate and Laird 1999). In addition, there are many related botanical products sold as health foods, food supplements, herbal teas, and for various other purposes related to health and personal care.