By Maurice Wibo (auth.), Jacques E. Dumont, Barry L. Brown, Nicholas J. Marshall (eds.)
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Natl. Acad. Sci. E. (1974), J. Mol. A. (1969), J. Am. Chern. , (1910), J. , 40, 190 (29) SCATCHARD, G. (1949), Ann. Y. Acad. , BURK, D. (1934), J. Am. Chern. P. (1970), Eur. Jo Biochern. M. (1971), Arch. Biochern. E. (1974), Biochirn. Biophys. L. (1969) in "The Role of Adenyl Cyclase and Cyclic 3',5'-AMP in Biological Systems", ed. W. RaIl, M. Rodbell, P. Condliffe. , FAYET, Go, LISSITZKY, S. (1974), Europ. J. Biochern. W. (1971), J. BioI. M. (1974) J. BioI. A. (1973), Biochern. Biophys. Res. , (1974), J.
Heterogeneity 3. Negative cooperativity 4. Positive cooperativity 5. Negative & positive cooperativity DISSOCIABLE RECEPTOR TWO-STEP RECEPTOR C. Glucagon 1971 1971 1974 1973 1974 Kono & Barham Cuatrecasas Lesniak et al. Lee & Ryan Verrier et al. Fat cells 1. Homogeneity Insulin TIGHTLY COUPLED RECEPTORS Examples of Recently Proposed Models of Hormone-Receptor Interaction B. A. M. E. DUMONT 38 If only a fraction of the total bound hormone is retained on the mi11ipore filter or trapped in a centrifugation pellet, the Scatchard graph is once again distorted : from its upward convexity, a false conclusion of positive cooperativity could be drawn (Figure 8).
Cell BioI. , (1974 b), Analytical study of microsomes and isolated subcellular membranes from rat liver. IV. Biochemical, physical, and morphological modifications of microsomal components induced by digitonin, EDTA, and pyrophosphate, J. Cell BioI. , (1966), La centrifuation en gradient de densite. , (1968), The large-scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with Triton WR-1339. Improved isolation procedures, automated analysis, biochemical and morphological properties of fractions, J.