Download Ciba Foundation Symposium 120 - Papillomaviruses: PDF

A multidisciplinary quantity featuring contemporary learn in relation to papillomaviruses and their organization with specific sorts of malignant lesions. offers up to date findings on their category and their molecular biology and cell-virus interactions, together with mode of access, amendment of genetic fabric and pathogenecity; their dating to varied different types of dermis stipulations; their organization with cervical carcinoma and carcinomas of the bladder and alimenatry tract, with descriptions of in vitro stories; and at last, their immunology and attainable healing brokers, e.g., interferon and vaccination. the one finished textual content at the topic, it brings jointly the paintings of the various such a lot sought after researchers within the box.

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J Virol53:955-965 Lusky M, Berg L, Weiher H, Botchan M 1983 Bovine papilloma virus contains an activator of gene expression at the distal end of the transcriptional unit. Mol Cell Biol3:1108-1122 Nakabayashi Y, Chattopadhyah SK, Lowy DR 1983 The transforming function of bovine papillomavirus DNA. Proc Natl Acad Sci USA 80:5832-5836 Rosl F, Waldeck W, Sauer G 1983 Isolation of episomal bovine papillomavirus chromatin and identification of a DNase-I hypersensitive region. J Virol46:567-574 Sarver N, Rabson MS, Yang Y-C, Byrne JC, Howley PM 1984 Localization and analysis of bovine papillomavirus type 1 transforming functions.

BPV-1 contains transcriptional enhancers which can act in a position-independent and orientationindependent manner to increase the transcriptional activity of a heterologous gene. One of these elements, which is located in a non-coding region of the genome. can be transactivated by a specific viral gene product encoded by the E2 open reading frame. Mutations which eliminate this trans-activation function also have a drama tic effect on transformation and on stable plasmid maintenance. 1986 Papillomaviruses.

To test whether the putative transforming products encoded by the E2 and E6 cDNA clones might have a cooperative effect in transformation, cotransformation experiments were performed. It was found that, when the class E2 and E6 clones were cotransfected onto mouse C127 cells, the frequency of focus formation was higher than when either clone was transfected independently, and the effects on transformation were more than additive, indicating synergy. The foci induced by cotransformation with the E2 and E6 cDNA clones appeared sooner and were larger than with either cDNA clone alone (Yang et a1 1985).

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