Download Carbohydrate-Based Vaccines: Methods and Protocols by Bernd Lepenies PDF

By Bernd Lepenies

This quantity summarizes present state-of-the-art equipment concerning carbohydrate-based vaccines, from the id of an acceptable carbohydrate antigen through the practise of glycoconjugate vaccines to the characterization of vaccine applicants for his or her use in pre-clinical and medical reviews. Written within the hugely winning Methods in Molecular Biology series layout, chapters contain introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, simply reproducible laboratory protocols, and key tips about troubleshooting and averting recognized pitfalls.

Authoritative and useful, Carbohydrate-Based Vaccines: tools and Protocols goals to be a worthwhile guide that may facilitate study within the box of carbohydrate-based vaccines.

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Any dye can be used as long as it meets the following criteria: excitation and emission wavelengths match with scanner; water soluble; compatible with glycans; can be easily washed off; minimum photobleaching over time. 2. We routinely use 8 μL per sample to print five batches of 30 slides. Less sample volume can be used depending on the number of slides, as long as evaporation problem is minimized. 3. Pause printing if pins are clogged. Use a forceps to move the pin up and down 20 − 30 times until pin moves freely.

Annu Rev Immunol 13:655–692 22. Landers C, Chelvarajan RL, Bondada S (2005) The role of B cells and accessory cells in the neonatal response to TI-2 antigens. Immunol Res 31:25–36 23. Weintraub A (2003) Immunology of bacterial polysaccharide antigens. Carbohydr Res 338: 2539–2547 24. Vos Q, Lees A, Wu ZQ et al (2000) B-cell activation by T-cell-independent type 2 antigens as an integral part of the humoral immune response to pathogenic microorganisms. Immunol Rev 176:154–170 25. López-Herrera G, Vargas-Hernández A, González-Serrano ME et al (2014) Bruton’s tyrosine kinase—an integral protein of B cell development that also has an essential role in the innate immune system.

With fluorescent scanner at PMT 600. tiff file for future data analysis. Choose slides with minimum defects for high quality results. 3. Assemble slide module over the slide to separate 16 printed arrays. The printed area of each array should be aligned in the center of each well. Be careful not to smudge or smear the array surface. 2 Blocking Glycan Microarray This process serves dual roles: blocking excess epoxide on slide surface and washing off print dye. 1. Prepare a fresh solution of blocking buffer: PBS with 3 % BSA (see Note 5).

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