Download Biological Aspects of Brain Tumors: Proceedings of the 8th by Mark A. Israel (auth.), Kazuo Tabuchi M. D. (eds.) PDF

By Mark A. Israel (auth.), Kazuo Tabuchi M. D. (eds.)

Growth task of mind tumors, metabolism of mind tumors, mind tumors and cytokines, drug resistance of mind tumors, oncogenes and anti-oncogenes, uncomplicated reports in mind tumor biology and treatment are offered during this book.

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Extra info for Biological Aspects of Brain Tumors: Proceedings of the 8th Nikko Brain Tumor Conference, Karatsu (Saga) 1990

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Structure of the amplified c-erbB gene in glioblastomas. EcoRI-digested DNAs of GL-3 and GL-S cells carry a high copy number (20-30 copies) of the EGF receptor gene. Additionally possible rearranged DNA fragments were detected in both GL-3 and GL-S among S-7 kilobases (kb) DNA fragments (Fig. 1). For localizing the rearranged DNA fragments in the EGF receptor gene, various portions of the EGF receptor cDNA were purified and used as probes. No abnormal bands were detected in the region downstream from the middle portion of the extracellular domain to the carboxy terminus.

In order to examine the fine 30 M. Shibuya et a\. structure of the amplified c-erbB gene in glioblastomas. EcoRI-digested DNAs of GL-3 and GL-S cells carry a high copy number (20-30 copies) of the EGF receptor gene. Additionally possible rearranged DNA fragments were detected in both GL-3 and GL-S among S-7 kilobases (kb) DNA fragments (Fig. 1). For localizing the rearranged DNA fragments in the EGF receptor gene, various portions of the EGF receptor cDNA were purified and used as probes. No abnormal bands were detected in the region downstream from the middle portion of the extracellular domain to the carboxy terminus.

Screening of the cDNA Library GL-5 cells were maintained in athymic nude mice by transplantation of a tumor piece. Total RNA was isolated from fresh-frozen GL-5 cells, and poly(A)+ RNA was purified by affinity chromatography using oligo-d(T) cellulose. A complementary DNA library was constructed in Agt11 via a unique EcoRI site and screened by plaque hybridization with a cloned EGF receptor cDNA, pE7, as a probe. DNA Transfection Assay DNA transfer into NIH3T3 cells was carried out by a method using polycation and dimethyl sulfoxide as described elsewhere [11].

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